Detection of human cytomegalovirus and Epstein-Barr virus in symptomatic and asymptomatic apical periodontitis lesions by real-time PCR

摘要

Objectives: Recent studies have investigated the occurrence of human cytomegalovirus and Epstein-Barr Virusin samples from apical periodontitis lesions and a role in the pathogenesis of this disease has been suggested.Because genotype distribution and seroprevalence of EBV and HCMV differ among populations, it is importantto determine the presence of these viruses in endodontic periapical lesions of different populations. The aims ofthis study were to determine the presence of HCMV and EBV DNAs in samples from Turkish patients with symptomatic and asymptomatic apical periodontitis lesions using real-time polymerase chain reaction method and toevaluate their presence in both symptomatic and asymptomatic apical periodontitis lesions.Study Design: Periapical samples were collected from 12 asymptomatic and 16 symptomatic periapical lesions inconjunction with apicectomy. HCMV and EBV DNAs were identified in the samples by real-time PCR. The chi-squared test with Yates's correction or the Fisher's exact test was used to analyse the significance of differences.Results: HCMV DNA was detected in 10 of the 16 (62.5%) symptomatic and in five of the 12 (41.7 %) asymptomaticperiapical study lesions. The EBV DNA was identified in seven of the 16 (43.7 %) symptomatic and three of the 12 (25%) asymptomatic periapical lesions. The difference in occurrence of HCMV and EBV DNA between symptomaticand asymptomatic periapical lesions was not statistically significant. (All comparisons have p > 0.05).Conclusions: Our findings suggest that HCMV and EBV is a frequent inhabitant of both symptomatic and asymptomatic apical periodontitis lesions of endodontic origin in Turkish population.